![]() ![]() The DNA insert must contain particular sequences at the end of the fragments compatible with the prepared vector. The source for a DNA fragment or “DNA insert” can be genomic DNA, complementary DNA, plasmid DNA, PCR product, or synthetic DNA. ![]() To begin with, you have to prepare some important materials for cloning: Things to consider before you clone are the availability of your cloning materials, the goal of your research (such as to sequence a DNA fragment or to produce a protein), and the difficulty level to perform your protocol. You can perform molecular cloning by incorporating a piece of DNA into a plasmid (a recombinant DNA) to make more identical recombinant DNA in a living host. It is a useful tool to study a gene, modify the gene, reintroduce the modified gene into the natural host or another host, or to produce protein. ![]() Molecular cloning is a basic technique in molecular biology and biotechnology laboratories. ![]()
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